玉米素和水杨酸对雨生红球藻(Haematococcus pluvialis)生长及虾青素积累的影响     

崔丹丹  杨柳  孙雪  邹昭敏  李亚鹤  张琳  徐年军 《海洋与湖沼》2018,49(3):682-691

虾青素是一种具有强抗氧化活性的类胡萝卜素,而雨生红球藻是天然虾青素的主要来源。本文以雨生红球藻(Haematococcus pluvialis)为材料,研究了植物激素玉米素和水杨酸对雨生红球藻的生长、虾青素含量及相关基因表达的影响。分别添加5种浓度的玉米素或水杨酸,结果发现0.05mg/L玉米素或25mg/L水杨酸处理5d后雨生红球藻虾青素积累最多。该浓度玉米素或水杨酸可显著提高光胁迫下藻细胞密度,最高分别达到3.4×10~5cell/mL和3.0×10~5cell/mL;同时玉米素与水杨酸组中虾青素含量显著上升,分别为1.7%和1.6%,比对照组分别增加29.2%和25.6%。玉米素缓解了高光逆境条件下光合作用基因——Rubisco大亚基(rbcL)及其活化酶(rca)、碳酸酐酶(ca)的下调表达,但对虾青素合成途径β-胡萝卜素酮化酶基因(bkt)的表达量没有显著影响;而水杨酸则相反,在胁迫后期不能缓解光合作用相关基因的下调表达,但可使bkt基因显著上调,最高可达对照组的2.5倍。本研究首次比较了玉米素和水杨酸对雨生红球藻生长和虾青素积累的影响,发现玉米素比水杨酸具有更好的促进雨生红球藻中虾青素积累的效果。  相似文献   

我国北方农牧交错带西段退耕对土壤保持功能影响研究     

殷小菡  孙希华  徐新良  张雪艳  陈德超 《地球信息科学学报》2018,20(12):1721-1732

退耕还林（草）等生态工程对区域用地结构及生态系统服务功能产生了重要影响。本研究基于RUSLE模型,并辅以遥感监测与GIS空间分析方法,对北方农牧交错带西段2000-2015年退耕状况及其引起的土壤保持功能变化分3个时段（2000-2005年、2005-2010年及2010-2015年）进行了探究。结果表明：北方农牧交错带西段地区2000-2015年耕地面积净减少1663.83 km²,以转为林地、草地、建设用地为主,其中耕地转林、草地净减少面积为1113.64 km²,草地和未利用地是新增耕地的主要来源;15年间土壤保持功能提升显著,退耕还林（草）工程的实施使土壤保持量增加了56.50×10⁴ t,2005-2010年由退耕所带来的土壤保持增加量在3段时期中最高;不同坡度等级的生态退耕引起的土壤保持增加量差别较大,总体随着坡度升高呈下降趋势,但在25°以上的陡坡耕地由退耕还林（草）带来的土壤保持效益又有所升高。研究对于评估北方农牧交错带西段地区实施退耕还林（草）等工程的生态效益具有重要意义,并能为区域生态保护与修复工程的建设规划提供科学依据。  相似文献   

不同固有频率比条件下圆柱双自由度涡激振动特性的数值研究   总被引：1，自引：1，他引：0  

翟云贺  康庄  宋儒鑫  孙丽萍 《海洋工程》2018,36(4):50-59

采用RANS方法,结合SST k-ω湍流模型,对不同顺流向与横顺流向固有频率的比值(即固有频率比,fnx/fny)条件下低质量比圆柱体的双自由度涡激振动进行了二维数值模拟。圆柱体的质量比为2.6,雷诺数范围为2 500~18 750,相应的约化速度范围为2~15,包括了经典试验中出现的整个锁定范围。通过研究发现,固有频率比是影响振动特性的重要参数,随着固有频率比的增加,响应幅值逐渐降低且向更高的约化速度偏移;在低约化速度范围内,固有频率比对顺流向和横流向振动之间的相位差以及升力频率有较大影响,从而得到各种不同偏向的8字形轨迹;最后对不同固有频率比条件下的尾涡模式进行了讨论,给出了对应不同约化速度时的尾涡模式。  相似文献   

多氯联苯(Aroclor 1242)胁迫下鲤鱼肝脏组织氧化应激   总被引：1，自引：0，他引：1  

王仁君  王建国  丁宁  孙萌  高配科 《海洋与湖沼》2018,49(6):1286-1293

本文在实验室条件下通过静态水质染毒方式在生理生化和转录水平上探究了我国重要经济鱼种鲤鱼(Cyprinus carpioio)对多氯联苯Aroclor 1242的氧化应激:鲤鱼肝脏组织抗氧化酶[超氧化物歧化酶(SuperoxideDismutase,SOD)和过氧化氢酶(Catalase,CAT)]活性、脂质过氧化产物丙二醛(Malonydialdehyde,MDA)含量、相关抗氧化酶编码基因(Cu/Zn-SOD、Mn-SOD、CAT)、应激蛋白HSP70、转录因子Nrf2和芳烃受体AhR2基因表达。结果表明:Aroclor 1242胁迫下,鲤鱼肝脏组织抗氧化酶活性表现出低浓度促进高浓度抑制的趋势;肝脏组织丙二醛含量显著升高,其中390μg/L暴露组鲤鱼肝脏组织MDA含量在第6d高于对照组92.1%(P0.01),表明肝脏组织发生脂质过氧化,膜系统受到损伤;鲤鱼肝脏组织中Cu/Zn-SOD、Mn-SOD、CAT、HSP70、Nrf2和AhR2编码基因表达出现不同程度下调。本文从酶学和基因转录水平上揭示了Aroclor1242胁迫下鲤鱼肝脏组织细胞的氧化应激。  相似文献   

苯并[a]芘(BaP)对褐菖鲉(Sebasticus marmoratus)肝CYP1A1酶活性、基因表达及蛋白表达的影响     

孙文静  王晓艳  祁鹏志  张建设  吴常文 《海洋与湖沼》2018,49(4):897-903

为了了解苯并[a]芘(BaP)对鱼类细胞色素P4501A1(CYP1A1)表达的影响,以褐菖鲉(Sebasticus marmoratus)为实验材料,采用体内实验,研究其在经过不同浓度(0.1、1、10、20、50mg/kg鱼体重量)的BaP诱导后,鱼体肝脏研究CYP1A1基因表达的情况,筛选出后续时间-效应实验中BaP注射的最佳浓度,研究BaP诱导6h、12h、1d、3d、7d后(质量浓度为20mg/kg鱼体重量)鱼体肝脏CYP1A1酶活性、基因表达和蛋白表达的情况。结果表明:剂量-效应实验中,20mg/kg鱼体重量为最佳浓度,此浓度下,基因表达在各组中变化最显著。时间-效应实验中,较空白对照组而言,染毒6h、12h和1d后,EROD酶活性显著增加。3d后开始下降,与对照组相比变化不大,7d后酶活性又发生上调。半定量RT-PCR结果表明,各染毒组与对照组相比,CYP1A1基因表达量都发生了上调,呈现先上升后下降的趋势。其中,6h和12h组相对表达量极显著增加,1d后开始下降且与3d和7d组相比变化不明显。Western blot结果表明,蛋白表达量在染毒12h后表现出显著的诱导效应,随着时间的延长略有回落,但与对照组相比仍有显著性差异。研究表明:BaP对褐菖鲉CYP1A1具有较强的诱导作用。一定质量浓度的BaP注射于褐菖鲉不同的时间后,能诱导褐菖鲉活体EROD酶活性、CYP1A1基因m RNA表达及蛋白表达,并随着时间的延长呈现先诱导后抑制的趋势。这说明BaP作为诱导剂对CYP1A1酶活性和蛋白表达的作用机制可能与调控CYP1A1的转录水平有关。  相似文献   

Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp. B-3 on gene expression in RAW264.7 macrophage cells     

ZHANG Pingping  LI Jiang  YU Leiye  WEI Jingfang  XU Tong  SUN Guojie 《海洋学报(英文版)》2018,(8)

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印度尼西亚北苏拉威西蓝碧海峡浮游动物种类组成及分布   总被引：1，自引：0，他引：1  

王彦国  陈小银  邢炳鹏  孙柔鑫  FITRIA Nurul  项鹏  王春光  林茂 《海洋学报(英文版)》2018,37(12):35-44

Based on oceanographic survey data in June 2012 in the Lembeh Strait, the zooplankton ecological characteristics such as species composition, individual abundance, dominant species and distribution were analyzed. The results showed that 183 species(including 4 sp.) had been recognized, most of them belonged to copepoda.Cnidaria followed with 43 species(including 1 sp.) were identified. The average abundance of zooplankton was(150.47±58.91) ind./m~3. As to the horizontal distribution, the abundance of the zooplankton was higher in the southern waters than in the northern waters. The dominant species in the study area were Lensia subtiloides,Sagitta enflata, Lucifer intermedius, Oikopleura rufescens, Diphyes chamissoni, Creseis acicula, Subeucalanus subcrassus, Temora discaudata, Aglaura hemistoma, Doliolum denticulatum, Canthocalanus pauper, Oikopleura longicauda and Nanomia bijuga. Zooplankton biodiversity indexes were higher in study area than previous study in the other regions. The findings from this study provide important baseline information for future research and monitoring programs.  相似文献   

Seismogenic Structures of the 2006 ML4.0 Dangan Island Earthquake Offshore Hong Kong     

XIA Shaohong  CAO Jinghe  SUN Jinlong  LV Jinshui  XU Huilong  ZHANG Xiang  WAN Kuiyuan  FAN Chaoyan  ZHOU Pengxiang 《中国海洋大学学报(英文版)》2018,(1)

The northern margin of the South China Sea, as a typical extensional continental margin, has relatively strong intraplate seismicity. Compared with the active zones of Nanao Island, Yangjiang, and Heyuan, seismicity in the Pearl River Estuary is relatively low. However, a ML4.0 earthquake in 2006 occurred near Dangan Island(DI) offshore Hong Kong, and this site was adjacent to the source of the historical M5.8 earthquake in 1874. To reveal the seismogenic mechanism of intraplate earthquakes in DI, we systematically analyzed the structural characteristics in the source area of the 2006 DI earthquake using integrated 24-channel seismic profiles, onshore–offshore wide-angle seismic tomography, and natural earthquake parameters. We ascertained the locations of NW-and NE-trending faults in the DI sea and found that the NE-trending DI fault mainly dipped southeast at a high angle and cut through the crust with an obvious low-velocity anomaly. The NW-trending fault dipped southwest with a similar high angle. The 2006 DI earthquake was adjacent to the intersection of the NE-and NW-trending faults, which suggested that the intersection of the two faults with different strikes could provide a favorable condition for the generation and triggering of intraplate earthquakes. Crustal velocity model showed that the high-velocity anomaly was imaged in the west of DI, but a distinct entity with low-velocity anomaly in the upper crust and high-velocity anomaly in the lower crust was found in the south of DI. Both the 1874 and 2006 DI earthquakes occurred along the edge of the distinct entity. Two vertical cross-sections nearly perpendicular to the strikes of the intersecting faults revealed good spatial correlations between the 2006 DI earthquake and the low to high speed transition in the distinct entity. This result indicated that the transitional zone might be a weakly structural body that can store strain energy and release it as a brittle failure, resulting in an earthquake-prone area.  相似文献   

发现于我国渤海辽东湾的海洋甲藻新种-渤海异冒藻(Heterocapsa bohaiensis sp. nov.)     

肖洁  孙娜  张议文  孙萍  李艳  庞敏  李瑞香 《海洋学报(英文版)》2018,37(10):18-27

A small armed dinoflagellate bloomed in the aquaculture ponds off the coast of Liaodong Bay, Bohai Sea of China,resulting in heavy mortalities of the cultured prawns(Penaeus japonicus) and larvae of Chinese mitten handed crabs(Eriocheir sinensis). The bloom-forming species was successfully isolated, and cellular morphology of the specimen was consequently investigated through light, fluorescent and electron microscopy. The small((14.4±1.6) μm in length) ellipsoid cells show typical Heterocapsa thecal plate arrangement(Po, cp, 5′, 3 a, 7′′, 6 c,5 s, 5′′′, 2′′′′). The episome is evidently bigger than the hyposome. One to three spherical pyrenoids are located above or beside the large elongated nucleus. The body scale is characterized by a triangle basal plate with one central upright and nine peripheral spines. Above all, Heterocapsa bohaiensis could be distinguished from other Heterocapsa species by the combination of the cell size, morphology, cellular structure and body scale. Sequence analyses of both ITS and LSU regions reveal the significant genetic divergence between H. bohaiensis and other established species in this genus, further supporting novelty of this species. Noticeably, different sample treatment methods resulted in morphological variation of the apical pore complex(APC) of H. bohaiensis, which needs to be taken into account in future study.  相似文献   

“蛟龙”号载人潜水器运行与保障信息管理系统设计与应用          下载免费PDF全文

高翔  孙超  丁忠军  宋宏  赵晟娅  祝普强 《海洋开发与管理》2018,35(11):19-26

2017年我国"蛟龙"号载人潜水器完成了为期5年的试验性应用,期间累计下潜100余次,形成和建立了科学完善的应用保障体系和专业的应用人才队伍。以"蛟龙"号试验性应用中运行与保障的实践经验为基础,针对其中设备与备品备件管理、运行作业管理、拆检总装管理、数据资料查询与利用等方面的重要需求,设计开发了一套"蛟龙"号载人潜水器运行与保障信息管理系统,通过信息化平台实现设备资源、作业成果以及经验积累传承之间的高效衔接与复用,最大限度地降低装备运行成本、提高作业效率、保障海上作业安全。文章提出的载人潜水器运维体系能够为我国其他行业或部门的载人潜水器、远程缆控机器人(ROV)、水下自治机器人(AUV)等重大海洋装备的运行与保障提供有益参考借鉴。  相似文献